Biochemical Assays
Curia performs a wide variety of biochemical assays to support drug discovery efforts in both the small and large molecule arenas. Our Assay team has a broad experience base traditional enzyme kinetic analyses and ligand-binding assays as well as cutting edge kinetic analysis utilizing Octet Biolayer Interferometry (BLI) utilizing a wide variety of readouts and technologies. The assay team leverages the full menu of Multimode Readers, Genetic Assay Technologies, and Analytical Instruments available across Curia. A variety of liquid handling technologies and platforms are available to support studies at a small scale as well as at full HTS scale.
Assay Type Highlights:
- Enzyme Activity and Kinetic Evaluation
- Binding Affinity Determinations
- Immunoassays
- Membrane Prep Ligand-binding Assays
- HTS/Structure activity relation (SAR) screening and Secondary screen potency determinations
- Full customization of workflow and assay formats available
Assay Format/Technology Highlights:
- Fluorescent/Luminescence: HTRF, FP, FRET, AlphaLISA, AlphaScreen, Glo, Luciferase
- ELISA-Based: Meso Scale Discovery ECL and traditional TMB
- Spectrophotometric Abs and Radiometric Readouts
- HPLC and LC/MS methodologies
- Full Custom Development; Discovery through cGxP Assay Support
Liquid Handling Highlights:
- Formulatrix Tempest Automated Dispenser Platform
- 96/384 Channel Pipettors from Integra Biosciences and Apricot Technologies
- Tecan d300e Digital Dispenser
- Labcyte Echo 550 (3rd Party Collaboration)
