Cell Engineering
The cell engineering team performs stable cell line development and clone isolation for the production of recombinant proteins utilizing viral transduction and transfection methodologies. The team has experience with a large variety of cell types, including CAR-T, and a host of other cell lines derived from various tissues. Transgene expression is analyzed by various methods, such as flow cytometry, western blot, and ELISA.
Key features of Cell Engineering Services
- Variety of cell types
- HEK293, CHO, A549, hematopoietic (T cells, including CAR-T)
- Hundreds of engineered cell lines created
- Target types
- Enzymes, antibodies, membrane/structural proteins, reporter proteins, etc.
- Antibiotic titration (kill curve) analysis
- Gene delivery
- Viral vector-mediated transduction
- Cationic lipid transfection
- Single-cell cloning
- Research cell banks
Curia’s CAR T-Cell Engineering Services
| Lentivius Production | T Cell Transduction | T Cell Characterization | T Cell Delivery |
|---|---|---|---|
| Lentivirus engineered and produced at Curia | Client T cells are transduced with lentivirus encoding CAR | Cells are characterized for T cell markers and CAR | Cells are expanded, cryopreserved and delivered. |
