Viral vector platforms include Baculovirus, AAV, and Lentivirus. The cell engineering team performs stable cell line development and clone isolation for the production of recombinant proteins utilizing viral transduction and transfection methodologies. The team has experience with a large variety of cell types, including CAR-T, and a host of other cell lines derived from various tissues.
Available Viral Vector Systems
| Baculovirus | Lentivirus | Adeno-Associated Virus |
|---|---|---|
| – Bacmid system – BIIC storage – Suspension culture – Rapid high titer production (2 weeks) |
– Third generation – Four-plasmid system – HEK293 production |
– Insect (Sf9; baculovirus) HEK293 helper-free system |
Comprehensive Viral Vector Services
| Vector Engineering | Cell Engineering | Upstream Production | Downstream Production | Analytics |
|---|---|---|---|---|
| – Vector design – Gene synthesis – Genetic cloning – Plasmid production |
– Utilize viral vectors – Variety of cell lines – Primary cells (e.g., CAR-T) |
– Insect (Baculovirus) – Mammalian – Adherent and suspension platforms – Scale-up production |
– Depth filtration – Chromatography – Affinity, IEX, SEC etc., – Ultracentrifugation – Diafiltration |
– Capsid titer (ELISA) – Genome titer (ddPCR) – Infectious titer – Purity (PAGE) – Full empty capsid (electron microscopy) – Aggregation (DLS) – Bioburden/sterility – Mycoplasma (PCR) – Host cell DNA/Protein |
