Mammalian Expression
Starting from plasmid and construction design services, Curia provides a variety of protein production and purification services using both CHO and HEK293 cell lines. Affinity tags such as Fc, albumin, poly-histidine (His), maltose-binding protein (MBP), small ubiquitin-related modifier (SUMO), and FLAG are commonly used to facilitate protein purification. Highly streamlined molecular construction and transient production provide a fast turn around time.
Workflow of Mammalian Transient Expression
| Molecular Construction & DNA Scale-up | Mammalian Transient Production | Protein Purification | Extensive Quality Assessment |
|---|---|---|---|
| Customized construction of expression vectors with various affinity tags | 7-day or 14-day CHO or HEK production platform ranging from 30 mL to 10 L+ | Fast affinity resin purification method is employed (Protein A/G, IMAC, Anti-CH1, and many more) | Quality assessment of purity, aggregation, sequence confirmation, endotoxin, etc,. |
Curia:
1. Provides customized services for the purification of tagless proteins. A combination of ion-exchange, hydrophobic interaction, and other chromatography methods are used to enrich and purify the target molecule from the starting material. See here for more details about custom protein purification.
2. Has an in-house proprietary vector system that ensures compatibility with Tuna293™ Process or TunaCHO™ Process for transient production at various scales in HEK293 or CHO cells.
3. Offers a full line of protein analytical and characterization services.
4. Also provides stable cell line development for proteins via the CHO-GSN™ platform
| Catalog # | Name | Timeline | Price |
|---|---|---|---|
| 1168 | 0.1 L transient production in HEK293 cells (Tuna293™ process) and Anti-CH1 affinity chromatography purification/Anti-IgM affinity chromatography purification | 2 weeks | $2,580 |
| 1176 | 0.1 L transient production in HEK293 cells (Tuna293™ process) and IMAC purification/Blue Sepharose/Immobilized Glutathione purification | 3 weeks | $2,750 |
| 1179 | 1.0 L transient production in HEK293 cells (Tuna293™ process) and IMAC purification/Blue Sepharose/Immobilized Glutathione purification | 3 weeks | $10,340 |
| 1355 | 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Anti-CH1 affinity chromatography purification/Anti-IgM affinity chromatography purification | 3 weeks | $3,620 |
| 1363 | 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and IMAC purification/Blue Sepharose purification/Immobilized Glutathione purification | 4 weeks | $3,570 |
| 1366 | 1.0 L transient production in CHO cells (TunaCHO™ extended 14-day process) and IMAC purification/Blue Sepharose purification/Immobilized Glutathione purification | 4 weeks | $12,590 |
| 1749 | 0.1 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Anti-CH1 affinity chromatography purification/Anti-IgM affinity chromatography purification | 2 weeks | $2,580 |
| 1759 | 0.1 L transient production in CHO cells (TunaCHO™ standard 7-day process) and IMAC purification/Blue Sepharose purification/Immobilized Glutathione purification | 3 weeks | $2,750 |
| 1762 | 1.0 L transient production in CHO cells (TunaCHO™ standard 7-day process) and IMAC purification/Blue Sepharose purification/Immobilized Glutathione purification | 3 weeks | $10,340 |
| 1798 | Molecular construction of Fc fusion expression vector, 0.03L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification (template provided) | 5-6 weeks | $3,060 |
| 1799 | Molecular construction of small peptide tag protein expression vector, 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and IMAC purification (template provided) | 6 weeks | $4,920 |
| 1800 | Molecular construction of Fc fusion expression vector, 0.03 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification (template provided) | 4-5 weeks | $2,440 |
| 1801 | Molecular construction of small peptide tag protein expression vector, 0.1 L transient production in CHO cells (TunaCHO™ standard 7-day process) and IMAC purification (template provided) | 5 weeks | $4,100 |
| 27120 | Molecular construction of Fc fusion expression vector, 0.03 L transient production in HEK cells (Tuna293™ standard 7-day process) and Protein A purification (template provided) | 5 weeks | $2,330 |
| 27308 | Molecular construction of small peptide tag protein expression vector, 0.1 L transient production in HEK cells (Tuna293™ standard 7-day process), IMAC purification (template provided) | 5 weeks | $3,880 |