Mammalian Expression
Starting from plasmid and construction design services, Curia provides a variety of protein production and purification services using both CHO and HEK293 cell lines. Affinity tags such as Fc, albumin, poly-histidine (His), maltose-binding protein (MBP), small ubiquitin-related modifier (SUMO), and FLAG are commonly used to facilitate protein purification. Highly streamlined molecular construction and transient production provide a fast turn around time.
Workflow of Mammalian Transient Expression
| Molecular Construction & DNA Scale-up | Mammalian Transient Production | Protein Purification | Extensive Quality Assessment |
|---|---|---|---|
| Customized construction of expression vectors with various affinity tags | 7-day or 14-day CHO or HEK production platform ranging from 30 mL to 10 L+ | Fast affinity resin purification method is employed (Protein A/G, IMAC, Anti-CH1, and many more) | Quality assessment of purity, aggregation, sequence confirmation, endotoxin, etc,. |
Curia
- Provides customized services for the purification of tagless proteins. A combination of ion-exchange, hydrophobic interaction, and other chromatography methods are used to enrich and purify the target molecule from the starting material. See here for more details about custom protein purification.
- Has an in-house proprietary vector system that ensures compatibility with Tuna293™ Process or TunaCHO™ Process for transient production at various scales in HEK293 or CHO cells.
- Offers a full line of protein analytical and characterization services.
- Also provides stable cell line development for proteins via the CHO-GSN™ platform
