Cell Line Development
Curia offers advanced methods to rapidly move programs from DNA to RCB in weeks utilizing methods to ensure clonality and maximize titer across CHO, HEK 293, and other mammalian cell lines.
Developed from the CHO-K1 parental cell line, Curia offers the CHO-GSNSM stable cell line expression platform for the production of the proteins and antibodies. The CHO-GSNSM platform provides a glutamine synthetase (GS) knockout cell line for the generation of a research cell bank (RCB) and future use as a manufacturing cell line for commercial products.
Curia’s CHO-GSN™ CHO-K1 GS knockout for stable cell line generation is:
- Well characterized providing the ability to enable a 12-month construct to clinic platform
- Compatible with chemically defined commercial media
- Has been used in 100+ pre-clinical programs and clinical trials
Curia’s Technological Approach
- Electroporation to ensure efficient delivery of desired genes of interest to the cellular fabric
- Single cell plating to isolate multiple high expressors
- Cellular imaging to monitor proliferation of a consistent monoclonal cell line
Features
- Stable transfections to CHO-GSN℠ and CHOZN® cell lines
- Proven stability >60 generations
- Single cell cloning and monitoring for production of stable research cell lines
- Documented progression of research to master cell bank implementation