Viral Vectors
The Curia Vector Technology group offers a comprehensive suite of viral vector and cell engineering services, which cover vector engineering, process development, scale-up production, and analytical development.
Viral vector platforms include Baculovirus, AAV, and Lentivirus. The cell engineering team performs stable cell line development and clone isolation for the production of recombinant proteins utilizing viral transduction and transfection methodologies. The team has experience with a large variety of cell types, including CAR-T, and a host of other cell lines derived from various tissues.
Available Viral Vector Systems
Baculovirus
- Bacmid system
- BIIC storage
- Suspension culture
- Rapid high titer production (2 weeks)
Lentivirus
- Third generation
- Four-plasmid system
- HEK293 production
Adeno-Associated Virus
- Insect (Sf9; baculovirus) HEK293 helper-free system
Comprehensive Viral Vector Services
Vector Engineering
- Vector design
- Gene synthesis
- Genetic cloning
- Plasmid production
Cell Engineering
- Utilize viral vectors
- Variety of cell lines
- Primary cells (e.g., CAR-T)
Upstream Production
- Insect (Baculovirus)
- Mammalian
- Adherent and suspension platforms
- Scale-up production
Downstream Production
- Depth filtration
- Chromatography
- Affinity, IEX, SEC etc.,
- Ultracentrifugation
- Diafiltration
Analytics
- Capsid titer (ELISA)
- Genome titer (ddPCR)
- Infectious titer
- Purity (PAGE)
- Full empty capsid (electron microscopy)
- Aggregation (DLS)
- Bioburden/sterility
- Mycoplasma (PCR)
- Host cell DNA/Protein
Brochure
Vector Services for Advanced Therapies
Explore our vector services that include process development, FTE programs and project management.
Case Study
Purity and identity characterization of AAV capsid particles by LC-MS methods
