Poster: Discovery and Characterization of Epitope-Diverse Mismatch Repair (MMR) Protein Antibodies

Huntington’s disease (HD) is a devastating neurodegenerative disorder driven by an inherited CAG trinucleotide repeat expansion (≥36) in the huntingtin gene. Genome- and transcriptome-wide association studies (G/TWAS) have illuminated the DNA mismatch repair (MMR) pathway components as critical modifiers of disease progression, including onset age of motoric symptoms. By fueling somatic instability and repeat expansion, MMR activity may hold the key to understanding—and ultimately altering—the trajectory of HD. To enable interrogation of this mechanism, we have developed a suite of highly-specific, high-affinity monoclonal antibodies (mAbs) targeting FAN1, MLH1, MLH3, MSH2, MSH3, PMS1, and PMS2. These human/rodent cross-reactive mAbs exhibit remarkable sequence diversity and recognize 5–12 distinct epitopes per target, providing new reagents to probe MMR biology. This next-generation antibody toolkit provides much needed reagents to develop sensitive immunoassays to quantitate and visualize the MMR proteins in biofluids, cells and tissues.

Download the full poster to learn how these high-affinity, epitope-diverse mAbs provide a powerful toolkit to dissect the role of DNA mismatch repair proteins in HD.

Discovery and Characterization of Epitope-Diverse Mismatch Repair (MMR) Protein Antibodies_Curia