Protein Expression & Analysis

Curia’s expert scientists use advanced technology to produce and analyze high-quality recombinant proteins. These proteins serve as custom assay reagents, protein standards, vaccine antigens, and tools to enhance our understanding of biology and pharmacology.

Protein Expression

• Construct design for any expression system
• Protein expression using bacterial (E.coli), insect cells (Sf9, Sf21 or Tni), mammalian cells (HEK293 or CHO) or yeast (P. pastoris or S. cerevisiae)
• Culturing and expression in shaking flasks, 0 – 50L
• Large-scale fermentation (up to 2000L) is available through Curia development sites

Protein Purification

• Purification using Cytiva™ ӒKTA Pure™ or ӒKTA Avant™ FPLC, Bio-Rad™ NGC or Waters™ Preparative HPLC systems
• Purification from cytosol, conditioned media or inclusion bodies
• Extensive experience producing enzymes, membrane proteins and particularly challenging proteins, for example Huntingtin (HTT) and Huntington’s disease (HD) related proteins

Protein Analysis

• SDS-PAGE, Western blot and ProteinSimple™ Jess™
• UHPLC-SEC-UV-MALS-RI
• Dynamic Light Scattering (DLS)
• RP-HPLC
• Endotoxin measurement by LAL assay
• Bioactivity assessment by biochemical, biophysical or ligand-displacement assay
• Protein thermal shift (PTS)
• Surface Plasmon Resonance (SPR) by Biacore™
• Intact mass by Sciex™ 6600 TripleTOF® LC-MS, Thermo Scientific™ – Orbitrap Exploris™ LC-MS or Bruker™ UltrafleXtreme MALDI-TOF/TOF MS
• Peptide mapping by Thermo Scientific™ – Orbitrap Exploris™ LC-MS
• High resolution structure determination of proteins or protein-ligand complexes by X-ray diffraction or cryo-electron microscopy (cryo-EM).