Antibody Reformatting & Production
Antibody variable region genes (VH and VL) from a given antibody can be reformatted to encode a different constant region, including Fab. Multiple species and isotypes are available, with the option of synthesizing the constant region of choice. These expression constructs are transiently transfected into HEK293 cells (Tuna293™ Process) or CHO cells (TunaCHO™ Process) at various scales followed by Protein A purification or other affinity-based chromatography of choice.
Pilot scale is shown below. For larger production scales, please inquire.
Click here to learn more about Curia’s gene synthesis and molecular cloning capabilities.
Intact mass spec analysis for all recombinant antibody productions.
| Catalog # | Name | Timeline | Price |
|---|---|---|---|
| 1133 | Molecular construction of IgG, 0.03 L transient production in HEK293 cells (Tuna293™ standard 7-day process), Protein A purification (template provided) | 4 weeks | $2,730 |
| 1134 | Molecular construction of IgG, 0.03 L transient production in HEK293 cells (Tuna293™ standard 7-day process), Protein A purification (synthesize variable regions) | 5 weeks | $3,230 |
| 1794 | Molecular construction of IgG expression vectors, 0.03 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification (template DNA provided) | 4 weeks | $2,840 |
| 1795 | Molecular construction of IgG expression vectors, 0.03 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification (synthesize variable regions) | 5 weeks | $3,340 |
| 1796 | Molecular construction of IgG expression vectors, 0.03 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification (template DNA provided) | 5 weeks | $3,460 |
| 1797 | Molecular construction of IgG expression vectors, 0.03 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification (synthesize variable regions) | 6 weeks | $3,960 |
