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Protein Chemistry Publications

  • LeMaster DM, Bashir Q, Hernández G. Propagation of conformational instability in FK506-binding protein FKBP12. (2024) Biochim Biophys Acta Proteins Proteom. 1872(3):140990.
  • Harris, M. S., Dolan, R. F., Bryce, J. R., Ewusi, J. G., & Cook, G. A. (2023) In Vitro Glycosylation of the Membrane Protein γ-Sarcoglycan in Nanodiscs. ACS omega, 8(43), 40904-40910.
  • Samrat SK, Bashir Q, Huang Y, Trieshmann CW, Tharappel AM, Zhang R, Chen K, Zheng YG, Li Z, Li H. (2023) Broad-Spectrum Small-Molecule Inhibitors Targeting the SAM-Binding Site of Flavivirus NS5 Methyltransferase. ACS Infect Dis. 9(7):1319-1333.
  • Samrat SK, Bashir Q, Zhang R, Huang Y, Liu Y, Wu X, Brown T, Wang W, Zheng YG, Zhang QY, Chen Y, Li Z, Li H. (2023) A universal fluorescence polarization high throughput screening assay to target the SAM-binding sites of SARS-CoV-2 and other viral methyltransferases. Emerg Microbes Infect. 12(1):2204164.
  • Williams D, Brzezinski W, Gordon H, De Luca V (2022) Site directed mutagenesis of Catharanthus roseus (+)-vincadifformine 19-hydroxylase (CYP71BY3) results in two distinct enzymatic functions, Phytochemistry, 201:113265
  • Dai, S., Venturini, E., Yadav, S., Lin, X., Clapp, D., Steckiewicz, M., Gocher-Demske, A. M., Hardie, D. G., & Edelman, A. M. (2022) Calcium/calmodulin-dependent protein kinase kinase 2 mediates pleiotropic effects of epidermal growth factor in cancer cells. Biochimica et Biophysica Acta (BBA) – Molecular Cell Research, 1869(7), 119252.
  • Pace JB, Huang NN, Seguin JP, Esquina C, Olin E, Zhu G, Carr G (2021) Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System, Journal of Visualized Experiments on December 10, (178).
  • Damle MS,. Singh AN, Peters SC, Szalai VA, Fisher OS (2021) The YcnI protein from B. subtilis ontains a copper-binding domain. J Biol Chem., 297(3), 101078.
  • Naz H, Gull S, Bashir Q, Rashid N, Shahzad N. (2021) Thermococcus kodakarensis-derived L-asparaginase: a candidate for the treatment of glioblastoma. Biologia. 76(4), 1305-1314.
  • Ahangama Liyanage, L., Harris, M. S., & Cook, G. A. (2021) In vitro glycosylation of membrane proteins using N-glycosyltransferase. ACS omega, 6(18), 12133-12142.
  • Bashir Q, LeMaster DM, Hernández G. (2020) 1H, 13C, 15 N chemical shift assignments of the FKBP12 protein from the pathogenic fungi Candida auris and Candida glabrata. Biomol NMR Assign. 14(1):105-109.
  • Arif M, Bashir Q, Siddiqui MA, Rashid N. (2020) Molecular characterization of a highly efficient and thermostable phosphoribosyl anthranilate isomerase from Geobacillus thermopakistaniensis. Protein Expr Purif. 166:105523.
  • Jamaleddine, M., Harris, M. S., Liyanage, L., & Cook, G. A. (2020) Expression, purification, and structural analysis of the full-length human integral membrane protein γ-sarcoglycan. Protein Expr Purif. 167, 105525.
  • Maqsood B, Basit A, Khurshid M, Bashir Q. (2020) Characterization of a thermostable, allosteric L-asparaginase from Anoxybacillus flavithermus. Int J Biol Macromol. 152:584-592.
  • Williams D, Qu Y, Simionescu R, De Luca V (2019) The assembly of (+)-vinadifformine- and (-)-tabersonine-derived monoterpenoid indole alkaloids in Catharanthus roseus involves separate branch pathways, The Plant Journal, 9(4):626-636.
  • Gama, S. R., Lo, B. S. Y., Séguin, J., Pallitsch, K., Hammerschmidt, F., & Zechel, D. L. (2019) C–H Bond Cleavage Is Rate-Limiting for Oxidative C–P Bond Cleavage by the Mixed Valence Diiron-Dependent Oxygenase PhnZ. Biochemistry, 58(52), 5271-5280.
  • Li H, Zhang X, Wu H-Y, Sun L, Ma Y, Xu J, Lin Q, Zeng D. (2019) Cu-64 Labeled ubiquitin for PET imaging of CXCR4 expression in tumor. ACS Omega, 4, 12432-12437
  • Arif M, Rashid N, Perveen S, Bashir Q, Akhtar M. (2019) Extremely stable indole-3-glycerol-phosphate synthase from hyperthermophilic archaeon Pyrococcus furiosus. Extremophiles. 23(1):69-77.
  • An P, Wu H-Y, Lewandowski TM and Lin Q. (2018) Hydrophilic azaspiroalkenes as robust bioorthogonal reporters. Chem. Commun., 54, 14005-14008
  • Aziz I, Rashid N, Ashraf R, Bashir Q, Imanaka T, Akhtar M. (2017) Pcal_0111, a highly thermostable bifunctional fructose-1,6-bisphosphate aldolase/phosphatase from Pyrobaculum calidifontis. Extremophiles.21(3):513-521.
  • Kuai X, Barraco C, Després C. (2017) Combining fungicides and prospective NPR1-based “just-in-time” immunomodulating chemistries for crop protection. Frontiers in Plant Science 8: 292772.
  • Murphy TF, Brauer AL, Johnson A, Wilding GE, Koszelak-Rosenblum M, Malkowski MG (2017) A Cation-Binding Surface Protein as a Vaccine Antigen To Prevent Moraxella catarrhalis Otitis Media and Infections in Chronic Obstructive Pulmonary Disease. Clin Vaccine Immunol., 24(9), 130-17.
  • Wiens, B., & De Luca, V. (2016) Molecular and biochemical characterization of a benzenoid/phenylpropanoid meta/para-O-methyltransferase from Rauwolfia serpentina roots. Phytochemistry, 132, 5–15.
  • Séguin, J. P., & Zechel, D. L. (2016) The Di-Iron Oxygenase PhnZ. Encyclopedia of Inorganic and Bioinorganic Chemistry, 1-11.
  • Naoum, R., Séguin, J. P., Trant, J. F., Frampton, M. B., Hudlický, T., & Zelisko, P. M. (2016) A chemoenzymatic route to chiral siloxanes. Tetrahedron, 72(27-28), 4027-4031.
  • Gharib G, Rashid N, Bashir Q, Gardner QT, Akhtar M, Imanaka T. (2016) Pcal_1699, an extremely thermostable malate dehydrogenase from hyperthermophilic archaeon Pyrobaculum calidifontis. Extremophiles. 20(1):57-67.
  • Bibi T, Perveen S, Aziz I, Bashir Q, Rashid N, Imanaka T, Akhtar M. (2016) Pcal_1127, a highly stable and efficient ribose-5-phosphate pyrophosphokinase from Pyrobaculum calidifontis. Extremophiles. 20(6):821-830.
  • Murphy TF, Kirkham C, Johnson A, Brauer AL, Koszelak-Rosenblum M, Malkowski MG (2016) Sulfate-binding protein, CysP, is a candidate vaccine antigen of Moraxella catarrhalis. Vaccine, 34(33), 3855-61.
  • Otsuka T, Kirkham C, Brauer A, Koszelak-Rosenblum M, Malkowski MG, Murphy TF (2015) The Vaccine Candidate Substrate Binding Protein SBP2 Plays a Key Role in Arginine Uptake, Which Is Required for Growth of Moraxella catarrhalis. Infect Immun., 84(2), 432-8.
  • Anand A, LeDoyt M, Karanian C, Luthra A, Koszelak-Rosenblum M, Malkowski MG, Puthenveetil R, Vinogradova O, Radolf JD (2015) Bipartite Topology of Treponema pallidum Repeat Proteins C/D and I: outer membrane insertion, trimerization, and porin function require a c-terminal β-barrel domain. J Biol Chem., 290(19), 12313-31.
  • Salim, V., Wiens, B., Masada-Atsumi, S., Yu, F., & De Luca, V. (2014) 7-deoxyloganetic acid synthase catalyzes a key 3 step oxidation to form 7-deoxyloganetic acid in Catharanthus roseus iridoid biosynthesis. Phytochemistry, 101, 23–31.
  • Frampton, M. B., Séguin, J. P., & Zelisko, P. M. (2014) Enzymatic Modification and Polymerization of Siloxane-Containing Materials. Bio-Inspired Silicon-Based Materials, 73-89.
  • Jones MM, Johnson A, Koszelak-Rosenblum M, Kirkham C, Brauer AL, Malkowski MG, Murphy TF, (2014) Role of the oligopeptide permease ABC Transporter of Moraxella catarrhalis in nutrient acquisition and persistence in the respiratory tract. Infect Immun., 82(11), 4758-66.
  • Hervás M, Bashir Q, Leferink NG, Ferreira P, Moreno-Beltrán B, Westphal AH, Díaz-Moreno I, Medina M, de la Rosa MA, Ubbink M, Navarro JA, van Berkel WJ. (2013) Communication between (L)-galactono-1,4-lactone dehydrogenase and cytochrome c. FEBS J. 280(8):1830-40.
  • Nisar MA, Rashid N, Bashir Q, Gardner QT, Shafiq MH, Akhtar M. (2013) TK1299, a highly thermostable NAD(P)H oxidase from Thermococcus kodakaraensis exhibiting higher enzymatic activity with NADPH. J Biosci Bioeng. 116(1):39-44.
  • De Wael K, Bashir Q, Van Vlierberghe S, Dubruel P, Heering HA, Adriaens A. (2012) Electrochemical determination of hydrogen peroxide with cytochrome c peroxidase and horse heart cytochrome c entrapped in a gelatin hydrogel. Bioelectrochemistry. 83:15-8.
  • Mayer, P. R., Huang, N., Dewey, C. M., Dries, D. R., Zhang, H., & Yu, G. (2010) Expression, localization, and biochemical characterization of nicotinamide mononucleotide adenylyltransferase 2. J Biol Chem, 285(51), 40387-40396.
  • Page, M., Richardson, J., Wiens, B., Tiedtke, E., Peters, C., Faure, P., Burness, G., & Stuart, J. (2010) Antioxidant enzyme activities are not broadly correlated with longevity in 14 vertebrate endotherm species. Age, 32, 255–270.
  • Jamil F, Gardner QA, Bashir Q, Rashid N, Akhtar M. (2010) Mechanistic and stereochemical studies of glycine oxidase from Bacillus subtilis strain R5. Biochemistry. 49(34):7377-83.
  • Bashir Q, Rashid N, Jamil F, Imanaka T, Akhtar M. (2009) Highly thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Thermococcus kodakaraensis. J Biochem. 146(1):95-102.
  • Page, M., Wiens, B., Peters, C., Faure, P., & Stuart, J. (2009) A Comprehensive Investigation Shows no Relationship Between Antioxidant Enzyme Activities and Animal Species MLSP in Heart and Liver, but Positive Correlations in Brain Tissue. In Free Radical Biology And Medicine (pp. S148–S149).
  • Volkov AN, Bashir Q, Worrall JA, Ubbink M. (2009) Binding hot spot in the weak protein complex of physiological redox partners yeast cytochrome C and cytochrome C peroxidase. J Mol Biol. 385(3):1003-13.
  • Koszelak-Rosenblum M, Krol AC, Simmons DM, Goulah CC, Wroblewski L, Malkowski MG, (2008) His-311 and Arg-559 are key residues involved in fatty acid oxygenation in pathogen-inducible oxygenase J Biol Chem., 283(36), 24962-71.
  • Robb, E., Page, M., Wiens, B., & Stuart, J. (2008) Molecular mechanisms of oxidative stress resistance induced by resveratrol: Specific and progressive induction of MnSOD. Biochem Biophys Res Commun, 367(2), 406–412.
  • Zhu H, Zhu G, Liu J, Zhang XC, Li G. (2007) Rabaptin-5-independent membrane targeting and Rab5 activation by Rabex-5 in the cell, Mol Biol Cell, 18(10): 4119-28.
  • Bashir Q, Rashid N, Akhtar M. (2006) Mechanism and substrate stereochemistry of 2-amino-3-oxobutyrate CoA ligase: implications for 5-aminolevulinate synthase and related enzymes. Chem Commun (Camb). 28;(48):5065-7.
  • Murphy TF, Brauer AL, Kirkham C, Johnson A, Koszelak-Rosenblum M, Malkowski MG, (2013) Role of the zinc uptake ABC transporter of Moraxella catarrhalis in persistence in the respiratory tract. Infect Immun., 81(9), 3406-13.
  • Huang N, Negi S, Szebeni A, Olson, MOJ (2005) Protein NPM3 interacts with the multifunctional nucleolar protein B23/nucleophosmin and inhibits ribosome biogenesis. J Biol Chem 280(7): 5496-5502.
  • Koszelak Rosenblum ME, Schmidt K, Freas J, Mastri M, Fay PJ., (2002) Cofactor activities of factor VIIIa and A2 subunit following cleavage of A1 subunit at Arg336. J Biol Chem. 277 (14): 11664-9.
  • Wakabayashi H, Koszelak ME, Mastri M, Fay PJ, (2001) Metal ion-independent association of factor VIII subunits and the roles of calcium and copper ions for cofactor activity and inter-subunit affinity, Biochemistry, 40(34), 10293-300.
  • Fay PJ, Mastri M, Koszelak ME, (2001) Factor VIIIa cofactor activity shows enhanced ionic strength sensitivity in the absence of phospholipid. Biochim Biophys Acta., 1548(1), 159-68.
  • Fay PJ, Mastri M, Koszelak ME, Wakabayashi H (2001) Cleavage of factor VIII heavy chain is required for the functional interaction of a2 subunit with factor IXA. J Biol Chem., 276(15), 12434-9.
  • Koszelak, ME, Huggind, CF, and Fay, PJ (2000) Sites in the A2 subunit involved in the interfactor VIIIa interaction, J Biol Chem., 275(35), 27137-44.
  • Srinivasan, J, Koszelak, M, Mendelow, M, Kwon, Y-G, and Lawrence, DS. (1995). The Design of Peptide-Based Substrates for the cdc2 Protein Kinase. Biochem. J, 309, 927-931.