Fermentation Research & Development

Innovating Fermentation Processes for Seamless Development and Manufacturing

Curia’s dedicated fermentation development team, based in León (Spain), combines state-of-the-art facilities with expert knowledge to deliver customized solutions, from development to manufacturing. Our track record showcases unparalleled quality and regulatory compliance. With a multidisciplinary approach and versatile feedback control systems, we are adept at tackling challenges from benchtop experiments to full-scale production.

• Microbial Strain Development: Conducted in cutting-edge labs with precision fermentation techniques.
• Process Optimization: Scale-up capabilities for preclinical trials or manufacturing (non-GMP and GMP for early clinical Phases 1 and 2).
• Proven Success: A variety of case studies highlight Curia’s ability to rapidly deliver robust, cost-advantaged processes, new intellectual property (IP), and novel natural products (microbial collection).

Scale-Up and Customization

With over 20 years of experience, Curia excels in isolating and purifying complex biological mixtures. Our infrastructure includes fermenters ranging from 1L to 15,000L, supported by continuous centrifuges, chromatography systems, and advanced monitoring tools.

Key Capabilities Include:

• Fermenters: Sizes from 1L glass to 15,000L stainless steel.
• Filtration: Tangential flow filtration (TFF; including MF, UF, NF) and filter press.
• Chromatography Columns: Glass (2L to 80L) and stainless steel (15L to 500L).
• Distillation and Analytical Tools: HPLC, UPLC, and LC-MS/MS for process evaluation.

Custom Fermentation Solutions:

Upstream Process (USP): Cell bank construction, strain screening, and fermentation optimization for bacteria, yeast, and fungi.
Downstream Process (DSP): Centrifugation, filtration, crystallization, and chromatographic purification.
Flexible Scale: From milligrams to kilograms, with approaches tailored to your strain and molecule.

Industrial Fermentation and Biotransformation

Curia excels in seamlessly integrating biocatalysis with advanced chemical synthesis, creating innovative and efficient processes that optimize both safety and scalability. By harnessing the strengths of microorganisms acting as highly efficient chemical factories alongside traditional chemical development, our multidisciplinary approach unlocks new intellectual property opportunities, reduces environmental impact, and enhances process safety. This integration not only streamlines large-scale manufacturing but also enables the development of cost-effective and sustainable solutions tailored to the unique needs of each project.

Applications in Biotransformation:

• Pharmaceutical APIs: Antibiotics, immunosuppressants, antifungals, cannabinoids, steroids and enzymes.
• Consumer Goods: Food and cosmetic ingredients, probiotics, and agricultural microorganisms.
• Specialized Products: Recombinant proteins.

Chromatography Purification Expertise:

• Mastery of chromatography (normal-phase, reverse-phase, ion exchange, etc.) ensures high-quality separations.
• Scale-up capabilities for large volumes with solvent-optimized processes.

R&D Capabilities:

• GMP manufacturing for early clinical Phases 1 and 2.
• Extensive work with GMO and natural microbial strains.
• Optimization of critical process parameters for diverse compounds.