Phage and Yeast Display
Curia offers phage and eukaryotic-based yeast display platforms for in vitro discovery and engineering of potent antibody candidates for therapeutic or diagnostic use. High-affinity functional antibodies are routinely selected against a wide range of conventional or challenging targets (e.g. GPCRs) using customized or in-licensed state-of-the-art libraries. Multiple antibody scaffolds (llama VHH, scFv, Fab, reformatted IgG) can be delivered upon request. Curia’s unparalleled in vitro antibody discovery capabilities can be bundled with unique lead optimization, characterization, and downstream large-scale GMP production, therefore providing the entire spectrum of early to late-stage antibody-drug generation services.
Comprehensive service includes:
- Strategy design
- Access to naïve (including XOMA031 and XOMA040 libraries with a full human repertoire), semi-synthetic, or immune libraries of scFvs, Fabs or llama VHH and custom library screening.
- Primary and secondary functional screens (multiple hits)
- Lead antibody characterizations:
- Kinetics and epitope binning using Octet® or Carterra® LSA
- Ligand blocking
- Cell assays
Curia’s Phage Display Antibody Discovery Services
|Strategy Design||Library Construction||Screening and Selection||1 or 2 degree Functional Screens||Lead Antibody Characterization|
|– Flexible workflow
– Parallel campaigns
|– Large and diverse
– Different antibody scaffolds
|– Immobilized, cell, or soluble antigens
– ELISA/FACS binding
– Preliminary kinetics
|– Cell-based assays
– Reporter assays
– Neutralization assays
|– lgG reformatting
– Kinetics & epitope binning
– Ligand blocking
Client Testimonial: Tri-Institutional Therapeutics Discovery Institute and LakePharma (now part of Curia): A Partnership in Antibody Discovery to Enable Innovative Therapeutics
Download Infographic: Unique VHH Antibodies from Diverse Yeast & Phage Display Llama Immune Libraries
Discovery of Potent, Functional Anti-BTLA, and Anti-TIGIT Monoclonal Antibodies using Hybridoma and Phage Display Platform webinar recording available NOW!
Click here to download the free eBook: Introduction to Antibody Display-based Libraries
Download our Antibody Discovery and Engineering Services Brochure by clicking here.
|1014||Synthetic Phage Library Antibody Discovery Campaign||3 to 5 months||Request|
|1015||Naive Phage Library Antibody Discovery Campaign||3 to 5 months||Request|
|1016||Custom Immune Phage/Yeast Antibody Discovery Campaign||4 to 6 months||Request|