N-terminal Sequencing
N-terminal Edman sequencing of proteins or peptides determines the N-terminal amino acid residues of purified protein and antibody samples. It can be useful to determine cleavage sites, to identify an unknown protein, to confirm the identity of a known protein, or to design oligonucleotide probes. The advantages of N-terminal sequencing include high sensitivity, with measurements down to the picomolar level. Proteins can also be sequenced in solution or on PVDF blots.
Key benefits:
1. High sequence coverage – Identify up to 15 amino acids
2. Fast turnaround: as fast as 3 days
3. High sensitivity – as small as picomoles
| Catalog # | Name | Timeline | Price |
|---|---|---|---|
| 60000 | Bioanalytical Setup Fee (may be applied to any bioanalytical assay containing <10 samples) | Request | |
| 64000 | SDS-PAGE, transfer to PVDF membrane, and Ponseau S staining | 1 Week | Request |
| 64005 | N-terminal Sequencing (5 residue) | 2 weeks | Request |
| 64010 | N-terminal sequencing (10 residue) | 2 weeks | Request |
| 64020 | N-terminal sequencing (15 residue) | 2 weeks | Request |