Title:
N-terminal Sequencing (5 residue)

Description:
N-terminal Sequencing (5 residue)

Scope:
The band(s) of interest is excised and submitted to an ABI 494 Protein Sequencer.
In the event, the N-terminus of the protein is blocked and no sequences are obtained, the full project price is still invoiced.
Note: the above-quoted prices are based on analysis using Curia’s standardized methods, proven to work for a majority of antibodies and proteins.

Deliverable:
Edman degradation cycle chromatogram with calls for each cycle

Material Required:
PVDF membrane (Coomassie Blue or Ponceau S stained) cutout of target bands
Please load at least 5 µg for SDS-PAGE transfer onto PVDF blot for submission
If liquid sample will be provided, the service SKU-64000 will be performed and will require 100 µL at > 0.5 mg/mL
Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to Curia for analysis.

Timeline:
2 Weeks

Price:
$500