Baculovirus Expression Vector System (BEVS)

The Baculovirus Expression Vector System (BEVS) is an established method within the scientific and industrial communities for generating large quantities of recombinant protein. BEVS is a eukaryotic system that utilizes the Autographa californica nuclear polyhedrosis virus (AcNPV) for high-level expression of recombinant proteins in Lepodoptera-derived insect cell lines, such as Sf9, Sf21, and High Five cells. Recombinant proteins produced using the system typically have correct folding, disulfide bond formation, and oligomerization, resulting in the production of proteins that are functionally similar to their native forms. The system can accommodate large DNA inserts, perform intron/exon splicing, and yield relatively high levels of recombinant protein (0.1 to 50% of total protein). Short affinity tags, such as polyhistidine, Flag, and Twin-Strep, are often fused to recombinant proteins to aid in detection and purification of proteins. After affinity capture, tags can be removed using recombinant proteases such as TEV, HRV3C, thrombin, or SUMOStar.

Key Features of Baculovirus Platform

– Suspension-based culture for virus production

– High titer virus production within 2 weeks starting from plasmid

– Rapid titer determination utilizing flow cytometry

– Upstream optimization for viral vector production

– DOE studies

– Optimization of culture parameters (e.g. cell density, MOI, virus ratios, incubation time, medium optimization)

– Cryopreserved BIIC cell banks for long term storage of baculovirus

Curia offers traditional and bacmid-based platforms for protein expression. Available platforms include Oxford Expression System flashBAC™, Bac-2-the-Future, and Thermo Fisher Bac-to-Bac®.

Case Study

Purity and identity characterization of AAV capsid particles by LC-MS methods.


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