Catalog #21480 - Custom Cloning
- Additional sequences, such as a peptide tag or protease cleavage site, can be added using PCR.
- Correct clones are identified by sequence analysis.
1. Recombinant plasmid from a miniprep. 2. Certificate of analysis.
1. DNA template (PCR product or gene within a plasmid). 2. 1-2 ug vector (Curia offers several vector options–please inquire). 3. Appropriate sequence information. 4. For gateway cloning, midiprep DNA or higher is needed for entry and destination vectors. If miniprep DNA is provided by client, Midiprep scale-up service (cat# 21602) will be included. Please complete and include a DNA Studio Order form with any vectors or plasmids you send to Curia.