N-terminal Sequencing

N-terminal Edman sequencing of proteins or peptides determines the N-terminal amino acid residues of purified protein and antibody samples. It can be useful to determine cleavage sites, to identify an unknown protein, to confirm the identity of a known protein, or to design oligonucleotide probes. The advantages of N-terminal sequencing include high sensitivity, with measurements down to the picomolar level. Proteins can also be sequenced in solution or on PVDF blots.