Catalog #1799 - Molecular construction of small peptide tag protein expression vector, 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and IMAC purification (template provided)

Title:
Molecular construction of small peptide tag protein expression vector, 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and IMAC purification (template provided)

Description:
Molecular construction of small peptide tag protein expression vector (template provided)

– DNA scale-up sufficient for 0.1 L production (1 DNA), includes DNA sequence confirmation

– 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process)

– IMAC purification, CE-SDS analysis QC is included

Scope:
Molecular construction only services:

Protein genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

Scale-up productions are available at an additional cost

Packages for molecular construction and small scale production:

1. Protein genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

2. Pilot small-scale transient transfection into HEK293 cells (Tuna293™ Process) or CHO cells (TunaCHO™ process) is performed, followed by the listed purification method to assess expression levels and protein yield.

Deliverable:
Molecular construction only services:

-plasmid DNA (if in a commercially available vector)

OR

-plasmid DNA used in scale-up productions at LakePharma

Packages for molecular construction and small scale production:

1. Purified protein

2. Study Report

3. Certificate of Analysis

Please note that plasmid DNA will not be provided if LakePharma’s vector system is utilized.

Material Required:
1. Sequence of VH and VL

2. DNA template (if DNA synthesis is not desired)

Timeline:
6 weeks

Price:
Request