Catalog #61220 - Sequence Confirmation by Trypsin and Chymotrypsin Digestion (2 digestion)

Title:
Sequence Confirmation by Trypsin and Chymotrypsin Digestion (2 digestion)

Description:
Sequence Confirmation by Trypsin and Chymotrypsin Digestion (2 digestion)

Scope:
1. The antibody/protein of interest is denatured, reduced and digested with one or more proteolytic enzymes, such as trypsin.

2. The resulting peptide mixtures are separated on a reverse-phase HPLC coupled to a mass spectrometer

3. The mass spectra is compared against the target protein sequence and the percent coverage is calculated.

Note: the above quoted prices are based on analysis using Curia’s standardized methods, proven to work for a majority of antibodies and proteins. Method development may be necessary for atypical proteins with unique properties for an additional fee.

Deliverable:
Study report.

Material Required:
≥ 200µg of purified protein at ≥ 1mg/mL
Target protein sequence
(Optional) 10mL blank buffer formulation
Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to Curia for analysis

Timeline:
1-2 weeks

Price:
$1,500