Catalog #63120 - N-glycan Analysis by LC-MS (Trypsin Digestion)

Title:
N-glycan Analysis by LC-MS (Trypsin Digestion)

Description:
N-glycan Analysis by LC-MS (Trypsin Digestion)

PTM analysis will identify relative quantities of glycans, not absolute quantities
Curia will perform PTM analysis with Trypsin Digestion.
The standard protocol for PTM analysis at Curia includes the below 9 glycans for analysis. If client wants other glycans tested, an additional charge may apply and client must let Curia know this before project initiation.
G0, G0F, G1, G1F, G2, G2F, Man5, G2FSA, G2FSA2

Scope:
1. The antibody/protein of interest is denatured, reduced, and digested with one or more proteolytic enzymes, such as trypsin.

2. The resulting peptide mixtures are submitted to a reverse-phase HPLC coupled to a mass spectrometer.

3. The mass spectra is compared against the target protein sequence and the glycans of interests are identified (if present).

Note: the above quoted prices are based on analysis using Curia’s standardized methods, proven to work for a majority of antibodies and proteins. Method development may be necessary for atypical proteins with unique properties for an additional fee.

Deliverable:
Study report.

Material Required:
≥ 100µg of purified protein at ≥ 1mg/mL
Target protein sequence
Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to Curia for analysis

Timeline:
2 week

Price:
$1,680