Protein Aggregation Analysis by SE-UPLC

Protein Aggregation Analysis by SE-UPLC

Column: ACQUITY UPLC Protein BEH SEC 200, 1.7 µm, 4.6 x 150 mm column
Mobile Phase: 50 mM Sodium Phosphate, 500 mM NaCl, pH 6.2
*Additional fee may apply if a different mobile phase is requested

1. Perform size-based separation of proteins/antibodies on an SEC column with UV detection

2. Detect and measure soluble aggregates by area per cent (if present).

Note: the above quoted prices are based on analysis using Curia’s standardized methods, proven to work for a majority of antibodies and proteins. Method development may be necessary for atypical proteins with unique properties for an additional fee.

Report with results.

Material Required:
100µL of purified protein at ≥ 1mg/mL
Expected molecular weight of target protein
(Optional) 10mL blank buffer formulation
Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to Curia for analysis

3 days