Catalog #1527 - High-throughput production service 96-well (Tuna293™ process)

Title:
High-throughput production service 96-well (Tuna293™ process)

Description:
High-throughput molecular construction (synthesize variable regions), DNA scale-up, 0.01 L transient production in HEK293 cells (Tuna293™ process), and Protein A purification

DNA sequence confirmation is included
Intact mass QC by mass spectrometry is included for standard human and mouse IgGs only

Scope:
Molecular construction of IgG expression constructs in Curia’s proprietary vector (synthesize variable regions)
DNA scale-ups (midiprep scale), including sequence confirmation
0.01 liter transient productions in HEK293 cells (HTP Tuna293™ process)
HTP Protein A purification
Intact mass spec QC (reduced and deglycosylated conditions), complimentary for standard human and mouse IgGs only

Deliverable:
All purified protein in standard buffer and unadjusted concentration obtained from 0.01 liter productions, frozen in 96-well deep well plate
Study Report
Summary of yields and aliquots
Mass Spec report

Additional service options:
Individual aliquots: $1200/plate

Material Required:
1. VH and VL sequences for 96 antibodies.

2. DNA template (if DNA synthesis is not desired)

3. Specified constant region for all 96 antibodies. Additional fees may apply for more than one heavy chain and one light chain.

Note: One constant region for heavy chain and light chain must be specified for all 96 antibodies.

Timeline:
5-6 Weeks

Price:
$105,600