Catalog #1134 - Molecular construction of IgG, 0.03 L transient production in HEK293 cells (Tuna293™ standard 7-day process), Protein A purification (synthesize variable regions)

Title:
Molecular construction of IgG, 0.03 L transient production in HEK293 cells (Tuna293™ standard 7-day process), Protein A purification (synthesize variable regions)

Description:
Molecular construction of IgG in Curia’s proprietary expression vector, 0.03 L transient production in HEK293 cells (Tuna293™ process), Protein A purification (synthesize variable regions)

– Includes heavy + light chain

– Includes DNA scale-up and DNA sequence confirmation

– CE-SDS analysis QC is included

– Intact mass QC by mass spectrometry is included

Scope:
Molecular construction only services:

Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

Scale-up productions are available at an additional cost

Packages for molecular construction and small scale production:

1. Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.

2. Pilot small-scale (0.03 liter) transient transfection into HEK293 cells (Tuna293™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield. Or

3. Pilot small-scale (0.03 liter) transient transfection into CHOcells (TunaCHO™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield.

Deliverable:
Molecular construction only services:

-plasmid DNA (if in a commercially available vector)

OR

-plasmid DNA used in scale-up productions at Curia

Packages for molecular construction and small scale production:

1. Purified IgG

2. Study report

3. Certificate of Analysis

Please note that plasmid DNA will not be provided if Curia’s vector system is utilized.

Material Required:
1. Sequence of VH and VL

2. DNA template (if DNA synthesis is not desired)

3. Sequence of complete IgG (if specific constant region required)

Timeline:
5 weeks

Price:
$3,129