Catalog #1134 - Molecular construction of IgG, 0.03 L transient production in HEK293 cells (Tuna293™ standard 7-day process), Protein A purification (synthesize variable regions)
Title:
Molecular construction of IgG, 0.03 L transient production in HEK293 cells (Tuna293™ standard 7-day process), Protein A purification (synthesize variable regions)
Description:
Molecular construction of IgG in Curia’s proprietary expression vector, 0.03 L transient production in HEK293 cells (Tuna293™ process), Protein A purification (synthesize variable regions)
– Includes heavy + light chain
– Includes DNA scale-up and DNA sequence confirmation
– CE-SDS analysis QC is included
– Intact mass QC by mass spectrometry is included
Scope:
Molecular construction only services:
Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.
Scale-up productions are available at an additional cost
Packages for molecular construction and small scale production:
1. Antibody genes are synthesized or amplified by PCR from a template provided by the client and cloned into our proprietary high expression mammalian vectors.
2. Pilot small-scale (0.03 liter) transient transfection into HEK293 cells (Tuna293™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield. Or
3. Pilot small-scale (0.03 liter) transient transfection into CHOcells (TunaCHO™ Process) is performed, followed by the listed purification method to assess expression levels and protein yield.
Deliverable:
Molecular construction only services:
-plasmid DNA (if in a commercially available vector)
OR
-plasmid DNA used in scale-up productions at Curia
Packages for molecular construction and small scale production:
1. Purified IgG
2. Study report
3. Certificate of Analysis
Please note that plasmid DNA will not be provided if Curia’s vector system is utilized.
Material Required:
1. Sequence of VH and VL
2. DNA template (if DNA synthesis is not desired)
3. Sequence of complete IgG (if specific constant region required)
Timeline:
5 weeks
Price:
$3,230