Stability in Plasma
Assay System:
- Blood plasma from desired species.
- Stability determined by measuring percent remaining of test compound at multiple time points during incubation in plasma.
Assay Conditions:
| Test Compound Concentration |
10 µM |
| Percent Organic Solvent in Incubation |
0.5% acetonitrile |
| Incubation Time |
0, 15, 30, 60, and 90 minutes |
| pH |
7.4 |
| Temperature |
37 °C |
| Positive Control |
Propantheline and/or Enalapril |
| Analysis |
LC-MS (LC-MS/MS if necessary) |
| Number of Repeats |
2 |
Deliverable: Half-life of test compound.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.
