Stability in Plasma

Blood plasma from desired species.
Stability determined by measuring percent remaining of test compound at multiple time points during incubation in plasma.

Test Compound Concentration	10 µM
Percent Organic Solvent in Incubation	0.5% acetonitrile
Incubation Time	0, 15, 30, 60, and 90 minutes
pH	7.4
Temperature	37 °C
Positive Control	Propantheline and/or Enalapril
Analysis	LC-MS (LC-MS/MS if necessary)
Number of Repeats	2

Deliverable: Half-life of test compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.