Stability in Blood

Assay System:

  • Fresh whole blood from desired species.
  • Human blood is mixed gender.  All other species are male.
  • Stability determined by measuring percent remaining of test compound at multiple time points during incubation in blood.

Assay Conditions:

Test Compound Concentration 1 µM
Percent Organic Solvent in Incubation 0.5% acetonitrile
Incubation Time 0, 15, 30, 60, and 90 minutes
Temperature 37 °C
Analysis LC-MS (LC-MS/MS if necessary)
Number of Repeats 2

Deliverable: Half-life of test compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.