Stability in Blood

Fresh whole blood from desired species.
Human blood is mixed gender. All other species are male.
Stability determined by measuring percent remaining of test compound at multiple time points during incubation in blood.

Test Compound Concentration	1 µM
Percent Organic Solvent in Incubation	0.5% acetonitrile
Incubation Time	0, 15, 30, 60, and 90 minutes
Temperature	37 °C
Analysis	LC-MS (LC-MS/MS if necessary)
Number of Repeats	2

Deliverable: Half-life of test compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.