Protein Binding, Equilibrium Dialysis – Brain Tissue
Assay System:
- Brain tissue (other tissues and biological fractions available upon request).
- Protein binding of test compound measured using Rapid Equilibrium Dialysis (RED) device.
Assay Conditions:
| Test Compound Concentration | 1 µM |
| Percent Organic Solvent in Incubation | 0.5% acetonitrile |
| Incubation Time | 4 hours |
| pH | 7.4, adjusted prior to incubation |
| Temperature | 37 °C |
| Positive Control | Propranolol |
| Analysis | LC-MS/MS |
| Number of Repeats | 3 |
| Brain Homogenate | Prepared in 4 volumes of phosphate-buffered saline (PBS) |
Deliverable: Percent bound of test compound and positive control compound.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.