Protein Binding, Equilibrium Dialysis – Brain Tissue
- Brain tissue (other tissues and biological fractions available upon request).
- Protein binding of test compound measured using Rapid Equilibrium Dialysis (RED) device.
|Test Compound Concentration||1 µM|
|Percent Organic Solvent in Incubation||0.5% acetonitrile|
|Incubation Time||4 hours|
|pH||7.4, adjusted prior to incubation|
|Number of Repeats||3|
|Brain Homogenate||Prepared in 4 volumes of phosphate-buffered saline (PBS)|
Deliverable: Percent bound of test compound and positive control compound.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.