Protein Binding, Equilibrium Dialysis – Brain Tissue

Assay System:

  • Brain tissue (other tissues and biological fractions available upon request).
  • Protein binding of test compound measured using Rapid Equilibrium Dialysis (RED) device.

Assay Conditions:

Test Compound Concentration 1 µM
Percent Organic Solvent in Incubation 0.5% acetonitrile
Incubation Time 4 hours
pH 7.4, adjusted prior to incubation
Temperature 37 °C
Positive Control Propranolol
Analysis LC-MS/MS
Number of Repeats 3
Brain Homogenate Prepared in 4 volumes of phosphate-buffered saline (PBS)

Deliverable: Percent bound of test compound and positive control compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.
*Human plasma is pooled gender. All other species are male. Other genders are available upon request. Please inquire/specify below.