PAMPA (Parallel Artificial Membrane Permeability Assay)

Assay System:

  • Lipid-infused artificial membrane.
  • Passive transport of test compound measured through permeability across a phospholipid membrane.

Assay Conditions:

Test Compound Concentration 100 µM
Percent Organic Solvent in Incubation 1% DMSO
Incubation Time 5 hours
pH 7.4
Temperature 20 °C
Positive Control Verapamil
Analysis UV Absorbance or LC-MS (LC-MS/MS if necessary)
Number of Repeats 2

Deliverable: Passive permeability (Pe), mass retention (R)

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.
You must select at least one detection method.