PAMPA (Parallel Artificial Membrane Permeability Assay)
Assay System:
- Lipid-infused artificial membrane.
- Passive transport of test compound measured through permeability across a phospholipid membrane.
Assay Conditions:
| Test Compound Concentration | 100 µM |
| Percent Organic Solvent in Incubation | 1% DMSO |
| Incubation Time | 5 hours |
| pH | 7.4 |
| Temperature | 20 °C |
| Positive Control | Verapamil |
| Analysis | UV Absorbance or LC-MS (LC-MS/MS if necessary) |
| Number of Repeats | 2 |
Deliverable: Passive permeability (Pe), mass retention (R)
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.