N-Acetylation Using Human Recombinant N-Acetyltransferases or Liver Cytosol
Assay System:
- Mammalian liver cytosol or major human recombinant N-acetyltransferases.
- Formation of N-acetylated metabolites of test compound measured upon incubation with liver cytosol or recombinant N-acetyltransferases.
- Position of acetylation in the test compound can be elucidated upon request.
Assay Conditions:
| Test Compound Concentration | 5 µM |
| Percent Organic Solvent in Incubation | 1% acetonitrile |
| Incubation Time | 30 minutes |
| pH | 7.4 |
| Temperature | 37 °C |
| Positive Control | Sulfamethazine |
| Analysis | LC-MS/MS |
| Number of Repeats | 3 |
Deliverable: Formation of N-acetylated metabolites (% of parent) and determination of acetylation site (optional).
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.