N-Acetylation Using Human Recombinant N-Acetyltransferases or Liver Cytosol

Assay System:

  • Mammalian liver cytosol or major human recombinant N-acetyltransferases.
  • Formation of N-acetylated metabolites of test compound measured upon incubation with liver cytosol or recombinant N-acetyltransferases.
  • Position of acetylation in the test compound can be elucidated upon request.

Assay Conditions: 

Test Compound Concentration 5 µM
Percent Organic Solvent in Incubation 1% acetonitrile
Incubation Time 30 minutes
pH 7.4
Temperature 37 °C
Positive Control Sulfamethazine
Analysis LC-MS/MS
Number of Repeats 3

Deliverable: Formation of N-acetylated metabolites (% of parent) and determination of acetylation site (optional).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.
Please choose at least NAT1 and/or NAT2.