Metabolic Stability, Liver Microsomes (Single Time Point)

Assay System:

  • Mammalian liver microsomes.
  • Metabolic clearance determined by measuring percent remaining of test compound at a single time point after incubation with liver microsomes.

Assay Conditions:

Test Compound Concentration 1 µM
Percent Organic Solvent in Incubation 0.5% acetonitrile
Incubation Time 0 and 30 minutes
pH 7.4
Temperature 37 °C
Positive Control Testosterone
Analysis LC-MS (LC-MS/MS if necessary)
Number of Repeats 2

Deliverable: Percent remaining of test compound after 30 min incubation with liver microsomes. Incubation time can be adjusted as needed, upon request.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.