Metabolic Stability, Liver Microsomes (Single Time Point)
Assay System:
- Mammalian liver microsomes.
- Metabolic clearance determined by measuring percent remaining of test compound at a single time point after incubation with liver microsomes.
Assay Conditions:
| Test Compound Concentration |
1 µM |
| Percent Organic Solvent in Incubation |
0.5% acetonitrile |
| Incubation Time |
0 and 30 minutes |
| pH |
7.4 |
| Temperature |
37 °C |
| Positive Control |
Testosterone |
| Analysis |
LC-MS (LC-MS/MS if necessary) |
| Number of Repeats |
2 |
Deliverable: Percent remaining of test compound after 30 min incubation with liver microsomes. Incubation time can be adjusted as needed, upon request.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.
