Metabolic Clearance, Mammalian Hepatocytes

Assay System:

  • Cryopreserved mammalian hepatocytes in suspension.
  • Human hepatocytes are pooled from 10 donors (mixed gender).  All other species are male.
  • Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with hepatocytes.

Assay Conditions:

Test Compound Concentration 1 µM
Hepatocyte Concentration 106 viable cells/mL
Percent Organic Solvent in Incubation 0.1% DMSO
Incubation Time 0, 15, 30, 60, 90, and 120 minutes
pH 7.4
Temperature 37 °C
Positive Control Testosterone and 7-hydroxycoumarin
Analysis LC-MS
Number of Repeats 2

Deliverable: Intrinsic clearance, (µL/min/106 cells), t1/2, and predicted blood extraction ratio (EH).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.