Metabolic Clearance, Mammalian Hepatocytes
Assay System:
- Cryopreserved mammalian hepatocytes in suspension.
- Human hepatocytes are pooled from 10 donors (mixed gender). All other species are male.
- Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with hepatocytes.
Assay Conditions:
| Test Compound Concentration |
1 µM |
| Hepatocyte Concentration |
106 viable cells/mL |
| Percent Organic Solvent in Incubation |
0.1% DMSO |
| Incubation Time |
0, 15, 30, 60, 90, and 120 minutes |
| pH |
7.4 |
| Temperature |
37 °C |
| Positive Control |
Testosterone and 7-hydroxycoumarin |
| Analysis |
LC-MS |
| Number of Repeats |
2 |
Deliverable: Intrinsic clearance, (µL/min/106 cells), t1/2, and predicted blood extraction ratio (EH).
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.
