Metabolic Clearance, Mammalian Hepatocytes
- Cryopreserved mammalian hepatocytes in suspension.
- Human hepatocytes are pooled from 10 donors (mixed gender). All other species are male.
- Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with hepatocytes.
|Test Compound Concentration||1 µM|
|Hepatocyte Concentration||106 viable cells/mL|
|Percent Organic Solvent in Incubation||0.1% DMSO|
|Incubation Time||0, 15, 30, 60, 90, and 120 minutes|
|Positive Control||Testosterone and 7-hydroxycoumarin|
|Number of Repeats||2|
Deliverable: Intrinsic clearance, (µL/min/106 cells), t1/2, and predicted blood extraction ratio (EH).
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.