Metabolic Clearance, Liver S9 Fractions
- Mammalian liver S9 fractions.
- Human S9 fractions are pooled from 10 donors (mixed gender). All other species are male.
- Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with liver S9 fractions.
|Test Compound Concentration||1 µM|
|S9 Protein Concentration||1.25 mg/mL|
|Cofactors||NADPH, UDPGA, and PAPS|
|Percent Organic Solvent in Incubation||0.5% acetonitrile|
|Incubation Time||0, 5, 10, 15, 30, and 45 minutes|
|Positive Control||Testosterone and 7-hydroxycoumarin|
|Analysis||LC-MS (LC-MS/MS if necessary)|
|Number of Repeats||2|
Deliverable: Intrinsic clearance, t1/2, and predicted blood extraction ratio (EH).
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.