Metabolic Clearance, Liver S9 Fractions
- Mammalian liver S9 fractions.
- Human S9 fractions are pooled from 10 donors (mixed gender). All other species are male.
- Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with liver S9 fractions.
|Test Compound Concentration
|S9 Protein Concentration
||NADPH, UDPGA, and PAPS
|Percent Organic Solvent in Incubation
||0, 5, 10, 15, 30, and 45 minutes
||Testosterone and 7-hydroxycoumarin
||LC-MS (LC-MS/MS if necessary)
|Number of Repeats
Deliverable: Intrinsic clearance, t1/2, and predicted blood extraction ratio (EH).
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.