Metabolic Clearance, Liver Microsomes

Assay System:

  • Mammalian liver microsomes.
  • Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with liver microsomes.

Assay Conditions:

Test Compound Concentration 1 µM
Microsomal Protein Concentration 1 mg/mL
Percent Organic Solvent in Incubation 0.5% acetonitrile
Incubation Time 0, 5, 10, 15, 30, and 45 minutes
pH 7.4
Temperature 37 °C
Positive Control Testosterone
Analysis LC-MS (LC-MS/MS if necessary)
Number of Repeats 2

Deliverable: Intrinsic clearance, t1/2, and predicted blood extraction ratio (EH).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.