Metabolic Clearance, Liver Microsomes

Mammalian liver microsomes.
Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with liver microsomes.

Test Compound Concentration	1 µM
Microsomal Protein Concentration	1 mg/mL
Percent Organic Solvent in Incubation	0.5% acetonitrile
Incubation Time	0, 5, 10, 15, 30, and 45 minutes
pH	7.4
Temperature	37 °C
Positive Control	Testosterone
Analysis	LC-MS (LC-MS/MS if necessary)
Number of Repeats	2

Deliverable: Intrinsic clearance, t_1/2, and predicted blood extraction ratio (E_H).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.