Detection and Identification of Reactive Metabolites, Liver S9 Fractions

Assay System:

  • Liver S9 fractions
  • LC-MS/MS detection of reactive metabolites of test compound using glutathione, KCN, or methoxyl amine as trapping reagents

Assay Conditions:

Test Compound Concentration 50 µM
Incubation Time 3 hours
pH 7.4
Temperature 37 °C
Positive Control Clozapine (for glutathione and KCN trapping);
MTEP (3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]pyridine hydrochloride) (for methoxyl amine trapping)
Analysis LC-MS/MS
Number of Repeats 2

Deliverable: Molecular weights and relative abundance of detected conjugates of test compound with glutathione, KCN, or methoxyl amine. Tentative structures of conjugates determined using MS/MS analysis are available upon request.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.
Please choose at least one of the trapping reagents.