Detection and Identification of Reactive Metabolites, Liver Microsomes
- Liver microsomes
- LC-MS/MS detection of reactive metabolites of test compound using glutathione, KCN, or methoxyl amine as trapping reagents
|Test Compound Concentration||50 µM|
|Incubation Time||3 hours|
|Positive Control||Clozapine (for glutathione and KCN trapping);
MTEP (3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]pyridine hydrochloride) (for methoxyl amine trapping)
|Number of Repeats||2|
Deliverable: Molecular weights and relative abundance of detected conjugates of test compound with glutathione, KCN, or methoxyl amine. Tentative structures of conjugates determined using MS/MS analysis are available upon request.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.