Detection and Identification of Reactive Metabolites, Liver Microsomes
Assay System:
- Liver microsomes
- LC-MS/MS detection of reactive metabolites of test compound using glutathione, KCN, or methoxyl amine as trapping reagents
Assay Conditions:
| Test Compound Concentration | 50 µM |
| Incubation Time | 3 hours |
| pH | 7.4 |
| Temperature | 37 °C |
| Positive Control | Clozapine (for glutathione and KCN trapping); MTEP (3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]pyridine hydrochloride) (for methoxyl amine trapping) |
| Analysis | LC-MS/MS |
| Number of Repeats | 2 |
Deliverable: Molecular weights and relative abundance of detected conjugates of test compound with glutathione, KCN, or methoxyl amine. Tentative structures of conjugates determined using MS/MS analysis are available upon request.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.