Cytotoxicity in Hepatocyte Suspensions
Assay System:
- Cryopreserved hepatocytes in suspension.
- Human hepatocytes are pooled from 10 donors (mixed gender). All other species are male.
- Cytotoxicity of test compound measured by comparing cell viability of hepatocytes incubated with test compound against vehicle control.
Assay Conditions:
| Test Compound Concentration | 8 concentrations ranging from 300-0.14 µM |
| Percent Organic Solvent in Incubation | 1% DMSO |
| Incubation Time | 4 hours |
| Incubation Conditions | 37°C, 5% CO2, and saturating humidity |
| Positive Control | Tamoxifen |
| Negative Control | Acetaminophen |
| Analysis | Luminescence |
| Number of Repeats | 3 |
| Hepatocyte Concentration | 106 viable cells/mL |
Deliverable: LC50 of test compound.
Test Compound Requirement: 2-3 mg dry powder or 100-200 µL of 30 mM DMSO stock solution.