Cytotoxicity in Hepatocyte Suspensions

Assay System: 

  • Cryopreserved hepatocytes in suspension.
  • Human hepatocytes are pooled from 10 donors (mixed gender).  All other species are male.
  • Cytotoxicity of test compound measured by comparing cell viability of hepatocytes incubated with test compound against vehicle control.

Assay Conditions: 

Test Compound Concentration 8 concentrations ranging from 300-0.14 µM
Percent Organic Solvent in Incubation 1% DMSO
Incubation Time 4 hours
Incubation Conditions 37°C, 5% CO2, and saturating humidity
Positive Control Tamoxifen
Negative Control Acetaminophen
Analysis Luminescence
Number of Repeats 3
Hepatocyte Concentration 106 viable cells/mL

Deliverable: LC50 of test compound.

Test Compound Requirement: 2-3 mg dry powder or 100-200 µL of 30 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.