Cytotoxicity in Hepatocyte Suspensions
- Cryopreserved hepatocytes in suspension.
- Human hepatocytes are pooled from 10 donors (mixed gender). All other species are male.
- Cytotoxicity of test compound measured by comparing cell viability of hepatocytes incubated with test compound against vehicle control.
|Test Compound Concentration||8 concentrations ranging from 300-0.14 µM|
|Percent Organic Solvent in Incubation||1% DMSO|
|Incubation Time||4 hours|
|Incubation Conditions||37°C, 5% CO2, and saturating humidity|
|Number of Repeats||3|
|Hepatocyte Concentration||106 viable cells/mL|
Deliverable: LC50 of test compound.
Test Compound Requirement: 2-3 mg dry powder or 100-200 µL of 30 mM DMSO stock solution.