CYP Phenotyping, Recombinant CYP Isoforms

Assay System: 

  • Individually expressed recombinant human CYPs (rhCYPs).
  • Human CYP isoforms responsible for metabolism determined by measuring disappearance of test compound in incubations with individual rhCYPs.

Assay Conditions: 

Test Compound Concentration 1 µM
Incubation Time 30 minutes
pH 7.4
Temperature 37°C
Analysis LC-MS/MS
Number of Repeats 2

Study Report: Percent of test compound remaining after incubation with individual rhCYPs.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.