CYP Phenotyping, Recombinant CYP Isoforms
Assay System:
- Individually expressed recombinant human CYPs (rhCYPs).
- Human CYP isoforms responsible for metabolism determined by measuring disappearance of test compound in incubations with individual rhCYPs.
Assay Conditions:
| Test Compound Concentration | 1 µM |
| Incubation Time | 30 minutes |
| pH | 7.4 |
| Temperature | 37°C |
| Analysis | LC-MS/MS |
| Number of Repeats | 2 |
Study Report: Percent of test compound remaining after incubation with individual rhCYPs.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.