CYP Phenotyping, Liver Microsomes

Assay System:

  • Human liver microsomes.
  • Human CYP isoforms responsible for metabolism of test compound determined by measuring metabolism suppression in the presence of CYP isoform-selective inhibitors.

Assay Conditions: 

Test Compound Concentration 1 µM
Incubation Time 30 minutes
pH 7.4
Temperature 37 °C
Positive Control CYP isoform-selective probe substrates
Analysis LC-MS/MS
Number of Repeats 2

Study Report: Percent of metabolism of test compound suppressed by CYP isoform-selective inhibitors.

Test Compound Requirement: 2-3 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.