Chemical Stability
Assay System:
- Standard test systems include Phosphate Buffered Saline (PBS) pH 7.4, Simulated Gastric Fluid (SGF), and Simulated Intestinal Fluid (SIF).
- Additional test systems available upon request.
- Stability determined by measuring percent remaining of test compound at multiple time points during incubation in selected media.
Assay Conditions:
| Test Compound Concentration | 10 µM |
| Percent Organic Solvent in Incubation | 1% DMSO |
| Incubation Time (PBS) | 0, 2, 4, 6, 8, 24, 48, 72 hours |
| Incubation Time (SGF, SIF) | 0, 15, 30, 60, and 90 minutes |
| pH | 7.4 (PBS), 1.2 (SGF), 5.0 (fed-state SIF), 6.5 (fasted-state SIF) |
| Temperature | 37°C |
| Positive Control | Verapamil and Tamoxifen |
| Analysis | LC-MS (LC-MS/MS if necessary) |
| Number of Repeats | 2 |
Deliverable: Half-life of test compound.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.