Blood/Plasma Partitioning

Assay System:

  • Fresh blood from desired animal species.
  • Partitioning of test compound measured between red blood cells (RBC) and plasma.

Assay Conditions:

Test Compound Concentration 10 µM
Percent Organic Solvent in Incubation 1% acetonitrile
Incubation Time 60 minutes
pH adjusted by 2-fold dilution of blood with PBS buffer prior to incubation
Temperature 37 °C
Positive Control Chlorthalidone
Analysis LC-MS/MS
Number of Repeats 3

Deliverable: RBC-to-Plasma ratio (KRBC/PL).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

Fresh (non-frozen) whole blood
*If you choose "Other", please use the comments section below to request your desired system.