- Fresh blood from desired animal species.
- Partitioning of test compound measured between red blood cells (RBC) and plasma.
|Test Compound Concentration||10 µM|
|Percent Organic Solvent in Incubation||1% acetonitrile|
|Incubation Time||60 minutes|
|pH||adjusted by 2-fold dilution of blood with PBS buffer prior to incubation|
|Number of Repeats||3|
Deliverable: RBC-to-Plasma ratio (KRBC/PL).
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.