Aqueous Solubility, Shake Flask
- Phosphate Buffer Saline pH 7.4 (PBS); other aqueous systems available upon request.
- Solubility measured by incubating test compound in PBS for 24 h, followed by determination of dissolved concentration against a calibration curve.
|Test Compound Concentration||100 µM|
|Percent Organic Solvent in Incubation||No organic solvent added|
|Incubation Time||24 hours|
|pH||7.4 or as requested|
|Positive Control||Verapamil and Tamoxifen|
|Analysis||UV/VIS Absorbance or LC-MS (LC-MS/MS if necessary)|
|Number of Repeats||2|
Deliverable: Solubility of test compound (µM).
Test Compound Requirement: 1-2 mg dry powder.