Aqueous Solubility, Kinetic

Assay System:

  • Phosphate Buffer Saline pH 7.4 (PBS); other aqueous systems available upon request.
  • Kinetic aqueous solubility measured by incubating test compound in PBS for 1 h, followed by determination of dissolved concentration against a calibration curve.

Assay Conditions:

Test Compound Concentration 100 µM
Percent Organic Solvent in Incubation 1% DMSO
Incubation Time 1 hour
pH 7.4 or as requested
Temperature Ambient
Positive Control Verapamil and Tamoxifen
Analysis UV Absorbance or LC-MS (LC-MS/MS if necessary)
Number of Repeats 2

Deliverable: Solubility of test compound (µM).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.

*If you choose "Other", please use the comments section below to request your desired system.
You must select at least one detection method.