Catalog #1922 - Gigaprep scale Endofree grade (<1 EU/mg) DNA production service (estimated yield of 6.0-10.0 mg)
Title:
Gigaprep scale Endofree grade (<1 EU/mg) DNA production service (estimated yield of 6.0-10.0 mg)
Description:
Gigaprep scale Endofree grade (<1 EU/mg) DNA production service (estimated yield of 6.0-10.0 mg)
Scope:
- Client-provided plasmid DNA is transformed into competent cells.
- Cells are cultured up to the desired scale.
- Plasmid DNA is purified and processed with appropriate endotoxin removal steps.
- Additional services for low endotoxin requirement (<10 EU/mg, <1 EU/mg), plasmid sequence verification, and/or restriction enzyme digestion are available for additional fee.
Note: Estimated yields based on typical production methods using Curia vectors. Actual yields may vary depending on the number of copies of the supplied vector. Purified plasmid DNA through the endotoxin-free process is typically less than 100 EU/mg. Please contact us for more information.
Deliverable:
Purified plasmid with Certificate of Analysis, agarose gel analysis, and endotoxin measurement.
Material Required:
- Either plasmid DNA (1-2 ug), or a plate with bacterial colonies.
- Information on antibiotic resistance of the plasmid.
- Information for sequence or restriction enzyme verification, if confirmation is needed (additional charges may apply).
Note: Please complete and include a sample submission form with the samples you send to Curia.
Disclaimer: We highly recommend working with Ampicillin/Carbenicillin or Kanamycin resistance for bacterial scale ups. Any other bacterial selection marker may require an extended timeline and/or extra charge.
Timeline:
7-10 Business Days
Price:
Request