CHO Stable Cell Line Development
Curia provides stable CHO cell line development services for diagnostic or therapeutic products.
Developed from the CHO-K1 parental cell line, Curia offers the CHO-GSN™ stable cell line expression platform for the production of proteins and antibodies. CHO-GSN™ is a glutamine synthetase (GS) knockout cell line for the generation of a research cell bank (RCB) and future use as a manufacturing cell line for commercial products. With multiple RCBs used for programs at the clinical stage, this platform offers clients a cell line with lineage documentation and a commercial use license for commercialization (royalty-free option available).
Curia provides the CHO-GSN™ platform as a CHO stable cell line development service using its proprietary knockout cell line, custom stable expression vectors, and glutamine synthetase (GS) selection technology platform.
|CHO-GSN Parental Cells||Proprietary Stable Expression Vectors||Proprietary Stable Cell Line Generation Know-how|
As a service, RCBs are generated under the following workflow:
|Stable Vector Construction||Stable Pool Generation||Stable Clone Generation||Stable Subclone Generation|
|Curia Proprietary Stable Expression Vectors
||CHO-GSN High Titer Clones
||CHO-GSN Monoclonal Cell Line
Final Research Cell Banks
- High titer clones selected
- Monoclonality ensured
- Tolerance to shear force and other bioreactor conditions tested
After the RCB is developed, Curia also offers characterization services, MCB generation, and process development services:
- Comprehensive Protein Characterization
- Generational Stability Study
- Gene Copy Number Determination
- Master Cell Bank Generation
- Upstream, downstream, analytical development, and formulation development
Download flyer: Large-scale Ab Production: Hybridoma or Recombinant?
Download brochure: GMP production of antibodies & proteins for critical assays and diagnostic applications – From Development to Manufacturing in 100 days