Metabolic Stability, Liver Microsomes (Single Time Point)

Assay System:

Mammalian liver microsomes.
Metabolic clearance determined by measuring percent remaining of test compound at a single time point after incubation with liver microsomes.

Assay Conditions:

Test Compound Concentration	1 µM
Percent Organic Solvent in Incubation	0.5% acetonitrile
Incubation Time	0 and 30 minutes
pH	7.4
Temperature	37 °C
Positive Control	Testosterone
Analysis	LC-MS (LC-MS/MS if necessary)
Number of Repeats	2

Deliverable: Percent remaining of test compound after 30 min incubation with liver microsomes. Incubation time can be adjusted as needed, upon request.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.