CYP Phenotyping, Liver Microsomes
Assay System:
- Human liver microsomes.
- Human CYP isoforms responsible for metabolism of test compound determined by measuring metabolism suppression in the presence of CYP isoform-selective inhibitors.
Assay Conditions:
| Test Compound Concentration | 1 µM |
| Incubation Time | 30 minutes |
| pH | 7.4 |
| Temperature | 37 °C |
| Positive Control | CYP isoform-selective probe substrates |
| Analysis | LC-MS/MS |
| Number of Repeats | 2 |
Study Report: Percent of metabolism of test compound suppressed by CYP isoform-selective inhibitors.
Test Compound Requirement: 2-3 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.